To assess how CSL deficiency affected the transition from in vitro culture to the in vivo tumor situation, we compared the transcriptome from in vitro culturing with that from tumors at early and late stages after xenografting, using the S3 technology to bioinformatically sort out the tumor (human) from the stromal (mouse) transcripts (Chivukula et al., 2015). This evidence concerns the gene RBPJ and neoplasm.