An immunocytochemical and an immunofluorescence assay of IFNγ, cell-associated IL-1α, and GM-CSF demonstrated their active production in granuloma cells (macrophages, multinucleate Langhans giant cells, and fibroblasts) from the spleens of mice after 20 days, one month, and two months following infection with the BCG vaccine in vivo and after ex vivo culture for 48–96 h (see Table 1, Figures 6(a)-6(b), 7(b)-7(c) and 10(c)). The gene discussed is IL1A; the disease is infection.