To bypass a predictable obstacle that Nrf1α-reading frameshift mutations are allowed to abolish the expression of TCF11 in addition to Nrf1α per se, thus it is better for us to have chosen the human hepatocellular carcinoma (HepG2) cells that express a major full-length protein of Nrf1α rather than TCF11 (which is also lost in the mouse30, 31, 45, 46, 54), in order to establish an Nrf1α-specific kockout cell line by using the TALEN system. The gene discussed is NFE2L1; the disease is hepatocellular carcinoma.