The real-time qPCR analysis revealed a markedly low abundance of total Nrf1 mRNA in poorly low-differentiated hepatocellular carcinoma (i.e. C, sampled from the carcinoma nodules), with tumour embolus being in vessels, and its para-carcinoma tissue (i.e. P, sampled within a more than 2-cm distance from the carcinoma nodules), and much lower level of mRNA expressed in the carcinoma compared to para-carcinoma (Fig. 14c, column #6, and S6f). This evidence concerns the gene NRF1 and neoplasm.