MLN and gastric cancer: After optimizing the appropriate concentration of CQ to be 5 μM for 72 h treatment, which was capable of blocking autophagy without affecting the growth of AGS/SGC-7901 cells (data not shown), we determined whether CQ could sensitize gastric cancer cells to MLN4924 by cell proliferation assay, and found that MLN-CQ combination attenuated IC50 of MLN4924 alone from about 80 nM to 40 nM in AGS cells and 150 nM to 80 nM in SGC-7901 cells, respectively (Fig. 4f).