To further corroborate Mito-Esc’s ability to protect from Ang-II-induced endothelial dysfunction during the progression of atheromatous plaque formation, phospho-eNOS, eNOS, phospho-AMPKα and AMPKα protein levels were measured by immunoblotting in total aortic tissue homogenate and also by immunohistochemistry. The gene discussed is AGT; the disease is endothelial dysfunction.