Adapting a tool designed to search protein interaction networks in cancer, we identified a small number of variants in genes within the AVSD trios, two of which (COL2A1 and COL9A1) were subsequently validated in burden testing of a separate replication cohort of 100 individuals at a statistically significant threshold. The gene discussed is COL9A1; the disease is familial atrioventricular septal defect.