TRIM44 and non-small cell lung carcinoma: Finally, to assess whether the metastasis and proliferation of NSCLC caused by TRIM44 resulted from activation of mTOR signaling, we transfected NCI-H1650 cells with pTRIM44 or the pDoubleEx-EGFP-N1 empty vector (pN1) and treated them (or not) with everolimus (an mTOR inhibitor).