Consistent with above findings, IS had no effect on glycolysis in Raptor knockdown sarcoma U2OS cells (Figure 3D–3F), but still inhibited glycolysis in Rictor knockdown sarcoma U2OS cells (Figure 3G–3I), indicating that the suppression of mTORC1 was involved in IS inhibition of glycolysis. Here, RICTOR is linked to sarcoma.