To confirm the role of GNG7 in cancer inhibition, an expression vector containing the GNG7 cDNA with GFP and 3 × FLAG tag fused at the C-terminus (GNG7-GFP-FLAG, or GNG7-GF) were transfected into U2OS and HeLa cells, the two cell lines that have significantly lower level of GNG7 compared to 293T. Here, GNG7 is linked to cancer.