To confirm that NHERF1 knockdown is sufficient to cause hydrocephalus, we injected 1-cell stage zebrafish (Danio rerio) embryos with antisense morpholino oligonucleotides targeted to the nherf1/slc9a3r1 initiation codon (NHERF1-MO). The gene discussed is NHERF1; the disease is Hydrocephalus.