Transfection of colon cancer cell lines with miRNA-21 led to increases in downregulation of programmed cell death protein 4 (PDCD4), transforming growth factor beta receptor 2 levels of beta-catenin, TCF/LEF activity, and expressions of c-Myc, Cyclin-D, which are increased in cancer stem cells (CSCs) and where these are accompanied by an increased sphere-forming ability in vitro and tumor formation in SCID mice [77]. This evidence concerns the gene PDCD4 and malignant colon neoplasm.