Using antibody-based technologies, over expression of protein budding uninhibited by benzidazoles 1 homolog beta (BubR1), mitotic arrest deficient-like 1 (Mad2), NF-kappaB-activating kinase, caspase 10, activator protein-1, alpha-actinin 4 (ACTN4), 67 kDa laminin receptor (67LR), COX-2, p53, secret protein acidic and rich in cysteine (SPARC), migration-stimulating factor (MSF), and vascular endothelial growth factor C (VEGF-C) were shown to be associated with ESCC [132–135]. Here, ACTN4 is linked to esophageal squamous cell carcinoma.