To benchmark this ratio score for the estimation of PTEN deletion in PC, we used 424 benign and malignant prostate formalin-fixed paraffin-embedded (FFPE) tissue samples, consisting of 339 radical prostatectomy (RPE) specimens, 28 castration resistant prostate cancers (CRPCs), 17 lymph node metastases, 11 distant metastases, and 29 benign prostatic hyperplasias (BPHs). This evidence concerns the gene PTEN and benign prostatic hyperplasia.