In this study, human lung cancer cell lines were used to investigate the effect of RAD001 on phosphorylation of eIF4E, the expression of key proteins in the AKT/mTOR signaling pathway such as AKT, p-AKT (S473), S6, p-S6 (S235/236), eIF4E and p-eIF4E (S209) were detected in six human NSCLC cell lines by Western blot. The gene discussed is MTOR; the disease is non-small cell lung carcinoma.