To understand the mechanism of apoptosis induced by CGP57380 alone and that of exerting synergistic apoptosis-inducing effects on NSCLC cells by combination of CGP57380 and RAD001, the key proteins known to be involved in regulation of the extrinsic or intrinsic apoptotic pathway (i.e., death receptors, Bcl-2 family members) were detected by Western blot. This evidence concerns the gene BCL2 and non-small cell lung carcinoma.