To explore whether restoration of ATOH8 expression reverse malignant phenotype of NPC cells, we stably introduced wild-type ATOH8 into mesenchymal-like EBV-negative CNE2 cells and transiently introduced wild-type ATOH8 into mesenchymal-like EBV-positive C666 cells. This evidence concerns the gene ATOH8 and nasopharyngeal carcinoma.