To further confirm that the expression level of ATOH8 was correlated with the EMT status of NPC, we determined the expression of LMP1, ATOH8, and EMT markers E-cadherin, vimentin, β-catenin, Twist1, Snail, Slug and ZEB1 using Real-Time PCR in 41 NPC clinical samples collecting from Sun Yat-sen University Cancer Center. The gene discussed is SNAI1; the disease is nasopharyngeal carcinoma.