To examine whether BDA-366 induces BCL2 conformational change by exposure of the BCL2 BH3 domain in MM cells, we treated human RPMI8226 and U266 cells with BDA-366 at concentrations of 0.25 and 0.5μM, and stained the cells with anti-BCL2 BH3 domain specific antibody [31]. Here, BCL2 is linked to Miyoshi myopathy.