TBX1 and posterior cortical atrophy: To explore the hypothesis that Brachyury could influence therapy response, we used the primary PCa 22RV1 and the brain metastatic PCa DU145 cell lines (both Brachyury-negative) previously modulated to overexpress Brachyury (pcBrachyury) and the bone metastatic PC3 cell line (Brachyury-positive) with Brachyury sh-mediated depletion (sh.Brachyury) (Supplementary Figure S1A) [21].