Considering that the spliced gp100mel47–52/40–42 epitope was identified in an unbiased reverse immunology approach and the fact that the PBMCs from approximately 50% of randomly chosen untreated melanoma patients contained CD8+ T cells that were stimulated by the gp100mel47–52/40–42 peptide suggested that generation of spliced epitopes is indeed a rather frequent event. This evidence concerns the gene CD8A and melanoma.