CD8A and melanoma: For proof of principal, we therefore tested whether HLA-A*03:01+ melanoma patients possessed CD8+ T cells directed against the gp100mel47–52/40–42 peptide (Fig. 5A) and/or the HLA-A*03:01-restricted spliced peptide gp100mel195–202/192 [RSYVPLAH][R] (Fig. 5B), which was previously shown to trigger a T cell response in melanoma patients18.