In order to determine the role of YB1 CTD in antiangiogenic signalling in breast cancer cells, cellular and secreted VEGF in Ad‐GFP‐YB1 CTD‐overexpressing SK‐BR‐3 cells were examined; and results demonstrated that YB1 CTD inhibited cellular and secreted VEGF expressions in SK‐BR‐3 breast cancer cells. The gene discussed is YBX1; the disease is breast cancer.