SK‐BR‐3 breast cancer cells were infected with Ad‐GFP or Ad‐GFP‐YB1 CTD for 48 h, and Ad‐GFP‐overexpressing SK‐BR‐3‐conditioned media (GFP‐CM) and Ad‐GFP‐YB1 CTD‐overexpressing SK‐BR‐3‐conditioned media (GFP‐YB1 CTD‐CM) were collected to detect secreted VEGF expression levels by ELISA. This evidence concerns the gene YBX1 and breast cancer.