According to our research, although C631T polymorphism in the SPO11 gene is not associated with idiopathic male infertility in the Iran; Kashan population, but in silico-analysis of SPO11indicated that the SNP could make fundamental changes in the protein and mRNA structure of SPO11. Therefore further studies with larger sample size and diverse ethnic populations are required to confirm the general validity of our findings. The gene discussed is SPO11; the disease is male infertility.