DGKI and colonic neoplasm: Four genes exhibited high expression in RKO compared to the other MSI colon cancer cell lines (CMTM3, DGKI, OPCML, and DLC1) whereas two genes had low gene expression in RKO (EPCAM and KLK10). DLC1 showed a fold change <2 in the microarray analysis but it was included because of its potential significance as a tumor suppressor in colon and liver cancers [28, 29].