The present study reports anemia and eosinophilia which is in agreement with the earlier reports of Saheb and Hafeez who documented significant decrease (p<0.05) in the hematological values of TEC, PCV and Hb and increased eosinophilic count (p>0.05) in ruminal amphistomiasis as compared to healthy buffaloes [19]. Here, GSTM1 is linked to Increased total eosinophil count.