GSTM1 and Increased total eosinophil count: Many scientists reported findings in accordance with the present study viz. Panda and Mishra [17] who reported reduction of hemoglobin content and erythrocyte count with eosinophilia in clinical pathological studies of the buffalo calf affected with amphistomosis, Singh et al. [18] also observed that there was a significant drop in TEC (p<0.05), Hb (p<0.01), and PCV (p<0.01) in biliary amphistomiasis and significant increase in eosinophilic count (p>0.01) as compared to the healthy animals.