Subjects with NAFLD had a significantly higher body mass index (BMI) and serum levels of fasting glucose, glycosylated hemoglobin (HbA1c), alanine aminotransferase (ALT), aspartate transaminase (AST), triglyceride (TG), fasting insulin, and homeostasis model assessment of insulin resistance (HOMA-IR), compared to those without NAFLD. The gene discussed is INS; the disease is metabolic dysfunction-associated steatotic liver disease.