To investigate the effects of miRNA expression on the Rituximab response in hard-to-transfect DLBCL cell lines, we first generated a lentiviral vector construct, pLV/miRCS-PE, with two expression cassettes allowing simultaneous expression of (i) a miRNA of interest expressed from the human U1 small nuclear RNA (snRNA) promoter and (ii) the eGFP reporter gene driven by a phosphoglycerate kinase (PGK) promoter (Fig 1a). This evidence concerns the gene PRKG1 and diffuse large B-cell lymphoma.