Furthermore, the reduction and mislocalization of TJ proteins of the KD blastocysts in ICC assay, and the remarkable increase in paracellular permeability in the TE epithelium of ADAM10 KD blastocyst in the FITC dextran uptake assay support the involvement of ADAM10 in TJ assembly/integrity. This evidence concerns the gene ADAM10 and intrahepatic cholangiocarcinoma.