In the present study, we aimed to investigate the expression of a panel of layer-specific markers covering all cortical layers (Satb2, Cux2, RORβ, ER81, Tbr1, SMI32, and NeuN) in cortical tubers of patients with TSC compared to perilesional cortex of the same patients, as well as to FCD type IIb or aged- and location-matched controls. This evidence concerns the gene CUX2 and tuberous sclerosis.