The proportions of neurons that were Satb2+ and subsequently corrected for the total amount of neurons showed a trend towards loss of Satb2+ neurons in lower cortical layers in TSC tissue (perilesional and tuberal; L5) compared to controls and FCD type IIb (Table 1, Fig. 2r, Additional file 1: Figure S1). The gene discussed is SATB2; the disease is tuberous sclerosis.