SIRT3 and hydrops fetalis: In addition, to validate the reduction in Sirt1 and Sirt3 gene expression, we measured protein levels of SIRT1 and SIRT1 (Supplemental Fig. 1a and b, respectively) to show that HF diets reduce significantly reduce sirtuin abundance, with the greatest effect being observed in the HF/HF offspring (p < 0.001 HF/HF vs C/C).