As shown in Figure 2D, overexpression of Gli2 increased luciferase activity driven by the full-length or the short BS1-containing FoxM1 promoter, but not the pFoxM1–(−2621 ~ −465) region that lacks Gli2 binding sites or the mutated BS1-containing FoxM1 promoter in HepG2 cells (left graph of Figure 2Di), whereas Gli2 knockdown markedly decreased this luciferase activity in HCC-LM3 cells (right graph of Figure 2Di). This evidence concerns the gene FOXM1 and hepatocellular carcinoma.