To further confirm that Slug inhibited cell proliferation and tumor formation of cervical cancer cells by trans-suppressing Akt1/p-Akt1 through both the p21/p27 proteins and the Wnt/β-catenin signaling pathway, first of all, the recombinant Akt1 plasmid was transiently transfected in the SiHa-Slug and HeLa-shControl cells, in which high level of Slug expression trans-suppressed the expression of Akt1/p-Akt1. The gene discussed is SNAI2; the disease is cervical cancer.