Using bisulfite sequencing, we observed that the overall methylation status were quite similar between the 5-aza-dC treated and untreated thyroid carcinomas cell lines, providing indirect evidences that methylation beyond this CpG region could be associated with decrease or lack of ABI3 expression in thyroid carcinomas cell lines and thyroid carcinoma samples. This evidence concerns the gene ABI3 and thyroid gland carcinoma.