At 48 hours after transfection of ETS-1 shRNA or transduction of the over-expression vector, the relative activity of caspase-3 of the bladder cancer 5637 cells, T24 cells and UMUC-3 cells were detected by thecaspase-3 enzyme-linked immunosorbent assay (ELISA) assay. This evidence concerns the gene ETS1 and urinary bladder carcinoma.