In the mice that developed NAFLD, a Western blot analysis (Fig 1B) confirmed that the final levels of total PRDX4 protein was highly expressed throughout the liver (significantly 2.1-fold more elevated) and intestine (significantly 5.4-fold more elevated) of the Tg mice, compared to the WT mice. This evidence concerns the gene PRDX4 and metabolic dysfunction-associated steatotic liver disease.