INS and Hypoglycemia: Treatment of control or STZ mice with insulin over the course of 1 week and provision of a bolus dose prior to cardiac excision to induce hypoglycemia (glucose < 100 mg/dL) resulted in a significant decrease in Lucifer Yellow dye spread throughout the ventricle (p < 0.03), as assessed by measuring the length of Lucifer Yellow dye spread from the site of furthest Alexa 647 spread (Figure 3).