With the introduction of three genes, large T antigen, telomerase reverse transcriptase (TERT), and Harvey rat sarcoma viral oncogene homolog v12 mutant (RAS (V12)), the engineered primary cell will extend life span, become immortalized, and finally gain tumorigenic capacity, which is considered to represent different stages of tumor cell transformation [33, 34]. The gene discussed is TERT; the disease is neoplasm.