DEFB103A and head and neck squamous cell carcinoma: To evaluate the expression of hBD3 in HPV-associated HNSCC specimens, we first genotyped high-risk HPVs using a multiplex PCR approach by amplifying E6 and E7 for each of 13 high-risk HPV types, 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 and 68, on genomic DNA extracted from 4 HNC specimens.