To elucidate whether mature hepatocytes can be the origin of ICC induced by Kras activation and Pten deletion, we crossed LSL-KrasG12D/+; Ptenflox/flox mice with Alb-CreERT2+ mice in which tamoxifen (TMX)-inducible CreERT2 was knocked into endogenous Alb locus36. The gene discussed is PTEN; the disease is intrahepatic cholangiocarcinoma.