PVR and infection: Temperature sensitivity of the CAVA backbone virus was confirmed when infections were left up to 14 days at 37°C, in multiple cell lines (Vero, L20B, Hela, SK-N-MC, Hek293), and its neuroattenuation in vivo was demonstrated (S2 Table) in CD155 transgenic mice, a susceptible model for poliovirus neurovirulence [39].