SQSTM1 and renal cell carcinoma: This idea was supported by immunoblotting of p53, which showed that high molecular weight polymers were only detected in the insoluble fraction after chloroquine treatment in RCC.2 Here, we tested the binding of three molecules, namely, TGase 2, p53, and p62, in ACHN (Figure 3a) and CAKI-1 (Figure 3b) cells.