We also compared the migration of PAO1-GFP to wounds of CF vs CFTR-corrected CF airway epithelia to test the role of CFTR in binding P. aeruginosa and CF epithelia incubated with acidic (characteristic of CF) or alkaline pH (characteristic of nonCF) [21,22] to determine whether altered pH affected bacterial chemotaxis or binding. Here, CFTR is linked to cystic fibrosis.