The aim of this study was to investigate how genistein differently modulates the intracellular redox status in human non-small cell lung cancer A549 cells and human normal lung fibroblast MRC-5 cells, identify the targets of genistein in the Nrf2-Keap1 pathway, and evaluate the radiosensitizing effect of genistein on A549 cells. Here, KEAP1 is linked to non-small cell lung carcinoma.