RHO and neoplasm: To explore if the Rho/Rho kinase pathway influences TRPV4KO EC mechanosensitivity towards ECM stiffness, we next cultured NEC and TRPV4KO EC on transglutaminase linked gelatin gels of varying stiffness (98, 370, and 2280 Pa; representing low, intermediate, and high stiffness, respectively) [12, 33], which mimic the stiffness of tumor ECM [34–35].