Furthermore, we found that galectin-9 has a novel function in immunological surveillance by regulating F-actin polarization in NK cells, and this regulation is associated with the activation of Rho/ROCK1 signaling.These results provide a foundation for the future exploration of a potential role for galectin-9 in the regulation of NK cell trafficking, potentially representing a new target for the immune treatment of colon cancer. Here, RHO is linked to malignant colon neoplasm.