In a second comparison of oligo-clonal and sub-clonal ASIP(+) tumors, parental B16-F10 cells alone, an equal number of a 1:1 mixture of B16-F10 and oligo-clonal B16-ASIP or sub-clonal B16-ASIP* cells were implanted subcutaneously (s.c.)into C57BL/6 mice and the effect of antagonism and inverse agonism of MC1R by ASIP on survival and tumor growth were measured. This evidence concerns the gene MC1R and neoplasm.