ASIP and neoplasm: One of the sub-clones showing high ASIP expression, designated B16-ASIP*, was marked with the red fluorescent protein mCherry (B16-ASIP-mChy) by a second lentivirus transduction, while parental B16-F10 cells were marked with green fluorescent protein (B16-GFP) to facilitate identification and enumeration of the two tumor cell types following implantation into mice.