The second unexpected finding from our studies was that although the ASIP-expressing tumors were generally smaller in size, mice implanted with either B16-ASIP-mChy cells alone, or with a 1:1 mixture of B16-GFP and B16-ASIP-mChy cells had significantly larger overall tumor burdens at the time of euthanasia than did mice with either a 4:1 mix or B16-GFP cells only. This evidence concerns the gene ASIP and neoplasm.