To further determine the effect of p.Glu116Lys on tumor growth and metastasis in vivo, cells with stably over-expressing MKK7-116Glu or MKK7-116Lys were injected into nude mice subcutaneously (both for A549 and L78 cell lines), and intravenously (for A549 cell line only), respectively. The gene discussed is MAP2K7; the disease is neoplasm.