Figure 10 shows results of thin layer chromatography (TLC) analysis of the final product (Figure 10A–10C), the structure of the target-specific chemoradiotherapeutic and imaging agent (Figure 10D), and results of analysis of cytotoxicity of agent conjugated to MTX alone; to MTX conjugated to peptides targeting recognition sequence Arg-Gly-Asp (RGD), CXCR4, and MMP; and MTX conjugated to dye in human C2984 osteosarcoma cells (Figure 10E). The gene discussed is CXCR4; the disease is osteosarcoma.