Although western analysis of β-actin in samples of U87 and A549 tumor protein loaded for western and zymographic analysis showed that a greater amount of U87 protein was loaded (Figure 6C2), the level of zymogen was much lower, and the level of active MMP was much higher in U87 than in A549 protein (Figure 6C3 and 6C4). The gene discussed is ACTB; the disease is neoplasm.