They included: (1) the BMIlow/Chollow condition; (2) abnormal serum AST level; (3) hypoalbuminemia (serum albumin level < 3.5mg/dL); (4) higher serum AFP (AFP > 400mg/dl); (5) higher Child-Pugh grading; (6) higher TNM stage grading; and (7) greater tumor size ( > 5 cm) (Table 4). This evidence concerns the gene AFP and Hypoalbuminemia.