We studied apoptosis mechanism of SFN on glioblastoma through the comprehensive expression of Bcl-2, Bax, Bad and cytochrome C. According to the results of the present study, SFN downregulated the expression of Bcl-2, upregulated the expression of Bax, Bad, and the proportion of Bax/Bcl-2 proteins, which indicated that Bcl-2 family proteins are involved in the SFN triggered apoptosis in U251MG cells. This evidence concerns the gene CYCS and glioblastoma.