In the significantly mutated genes, we determined that KRAS (G13D), PIK3CA (E545), TP53 a splicing site mutation, APC (E854fs), KMT2C (E141G, K339N, P309S, and Y816_I817delinsX), SETBP1 (Q1558L), and NCOR1 (S63L) were ubiquitous mutations, whereas CDKN2A (D74A), SMAD4 (W168X), NCOR1 (N208K), KMT2C (G315C and K306fs) and INHBA (V58I) were specific to the primary cancer. This evidence concerns the gene SMAD4 and cancer.