After hanging drop culture for 1, 2, and 3 days, RT-PCR was used to determine the expression levels of cytokines, including insulin growth factor 1 (IGF-1), interleukin-6 (IL-6), and hepatocyte growth factor (HGF), which play important roles during inhibition of hepatic fibrosis. This evidence concerns the gene IL6 and Hepatic fibrosis.