TNF and Insulin resistance: To further analyse the changes of miR‐20a‐5p in a cellular model of insulin resistance, murine NCTC 1469 and Hep1‐6 hepatocytes were stimulated with 33.3 mmol/l glucose for 48 hrs, 0.25 mmol/l palmitate for 24 hrs, 10 nmol/l IL‐6 or 10 nmol/l tumour necrosis factor (TNF)‐α for 24 hrs respectively.