HSPA5 and non-small cell lung carcinoma: Part I also contains two chambers to separate A549 and SPCA-1 cells from WI38 cells in chamber B. After WI38 transformed into CAF in co-culture in part I, we cultured NSCLC (A549 and SPCA-1) cells as control group and GRP78 knockdown NSCLC cells as siRNA experimental group in Chamber C. After cells adhered to the chamber, secretion from above flowed into the secretion chamber.